miR-652-3p通过靶向Nptn抑制胶质母细胞瘤的增殖和迁移

目的探讨miR-652-3p对胶质母细胞瘤(GAM)增殖和迁移的影响。方法用miR-652-3p模拟物转染胶质母细胞系C6和U87，然后分别通过CCK8、细胞流式、EdU、Transwell和划痕实验研究miR-652-3p对细胞增殖和迁移的影响。利用实时定量聚合酶链反应（qRT-PCR）、蛋白质免疫印迹（Western Blot）和双荧光素酶报告基因实验检测miR-652-3p与Neuroplastin(Nptn) 的互作关系。通过CCK8、细胞流式、EdU、Transwell和划痕实验研究Nptn对细胞增殖和迁移的影响。结果miR-652-3p的过表达可以抑制C6和U87细胞的增殖和迁移；双荧光素酶报告基因实验结果表明miR-652-3p可以与Nptn结合，Nptn表达水平受到miR-652-3p水平的影响；干扰Nptn的表达可以抑制C6和U87细胞的增殖和迁移。结论miR-652-3p通过靶向Nptn抑制GBM的增殖和迁移。

Objective To investigate the effect of miR-652-3p on glioblastoma(GBM) cell lines. Methods GBM C6 and U87 cell lines were transfected with miR-652-3p mimic, and then the effects of miR-652-3p on cell proliferation and migration were detected by CCK8, flow cytometry, EdU, Transwell and wound-healing assays, respectively. The interaction between miR-652-3p and neuroplastin(Nptn) was detected by quantitative real-time polymerase chain reaction(qRT-PCR), Western blot and dual luciferase reporter gene assays. The effects of Nptn on cell proliferation and migration were investigated by CCK8, cytometry, EdU, Transwell and wound-healing assays. Results Overexpression of miR-652-3p inhibits the proliferation and migration of GBM cells. Nptn is a functional target of miR-652-3p. Interfering with the expression of Nptn inhibits the proliferation and migration of GBM cells. Conclusion miR-652-3p suppresses GBM proliferation and migration by targeting Nptn.